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Human element [ hs1889 ]


Position: chr3:122,690,302-122,695,291 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: SEMA5B - PDIA5

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
CAGCCTTGAGAAGGGAGATGtggcaggtgataattctgcttcaggtaattgatcggaaat
gttttccaaatccatcatggggcatgaagcaaagatattaacaggtctgccaagagccag
caagaataaggtcttatttacctctggatcctcagcccgtgtctattgaatgaatgaaag
tgagtctctcctcctgaatgcccagtggggaaggtgttcacacacttattgctgcatggg
tgctggtgggcctctgcccaaggagccttccccaaggctttctggaaggcaggtgcggca
cggtgagaagagcatggacttggaatgggaagccatggtgcaagccctgatgtcagccac
tcctgaactcgtcaatagacatgattccagtgggagcaacaggcaccgtgttctgcatcc
ttgggccccgctgcctcccttctctttgaaacagagagaataatgatagcaacaacaaca
gcaatcataacaacttcttcacagacttgtgaaggtgagatacttgtgcactgagagcgg
aacaagcactcaacaaacgcttgttgtccacatggatccgctgatcgatccaccatggct
gggtcacttctccagtgtggagagttttgaggagtccctcactgctccaggtaggaagct
ctgccactacctgacttacttttcctcttgctgcaacttaaaccctcttactccactgtc
tgtggtgacaaggaataggccgacagttggtgaaagctctctctaacactggtctcccct
gcttttccaggaaagtagcagaaacagactttttggagtcaggaactaatgttatggaga
gtgtgcagtgacttaagatgtatctaaatcagtgcaactccaagccacatggtaacacaa
atggttctgctgcccatgtataaagcatgccagcccttttccctggcatgtggcacactc
cagatctctcccctcatccagttatgtgtgtgcaaaactcagcctggatcagtcaccctt
gaaatatgagtcaaataaaattaaaattaaaaattgttcttgaatcacaccctgttttgc
attatctgtgctattatttgactttactcagccctttaaccactggctccagatgatccc
acagatgtaactgggccagcactggggagagagacccccacctgagtcggtaagagctgt
tgggatgctgcgctgggtgcagcataggtgaccagcagaaaggtagtgtcaggaatcatg
caaatccccttcatgtggatgggtccttgctactcttcacaaccacgatgccatcagatt
ccctgtgaagtctgcagaaggcttgtcatggctcccatttaactgatgagaagccagagg
ccctgagagcagctacagatccaaatgctcagagtgggaagttagcccaggtctgccttc
ccaccccgcagcctatccaggctcccagaaacactcttattccccaagtcaagccgtgat
ggctcacactggataaggtccccagccactgggagctgggagcctggaaactgtgggaga
acccctacagattaatgacaggtcattcagagtgtctatacaccttgttttggaaatcta
agaggtaaccatagttagatcccaaggtctccagaaaaatcactcataatactctctttg
gggagtgggcagtattgagactggggcaggaaagagaaatgatggactatcatcccagtc
tgagcccctgactccagcctgtgttcaggctatgtttatgtccttctctccctttacacc
cctagcttctagtctaggtgccaagtggccctgggcctctagtgccctgaggagaggcag
ccaatgagctgctaacccagaggacataagctagtccaactcagcctctgccatgtgacc
agccaaaagcagaacctcaaacccccaccccaagatgagtggggtccagcctggcgggca
gtggagggtgccgcagtctgctccctttcctcttttagagaggaaaaaagaaaatgagtg
aggaggggtggaacgtcaacagagaccagaagggaataaagaacactgaggccagttcat
ctgtgaaggtcgtgaaatggacagagttttgttccttttggtttatgttatcgcagttat
gatggtgtttgtgcagtaagtgaaacagtgaagggaaagtgtctggggtctgtgttggag
ctgaacgatgctgcaaacttggggggttgtagattcccaaggagggcaggcaatgtggag
cctccctgtcccactctgggaatctgcaacccaccaagtgtggagcccaggacagagggg
cagaggtatgggagtggggaagggcagagagggaggagaaggtagggagaaattccttaa
accctttcaggatttccttctggagggctgtgagtcttcttcaccaggcccaatacctgg
tactctgggtcctgccagctcagatgcctgaacttccagaacataattgtgttccagctc
agcagggcacagcctcgggccgtctaggagcaggctgccgttccccgaatgtacaccctg
tcagtgcccagatccatctggagagcaaatggctggctgaggggagagagagcccaggca
caccaccccccatctgctcccgccatgtgacacatgcttgcaggcggggagggagaagcc
ggcaggtggttggctgatgtagagtggaggctgggagaaagaacaaggaccagcacaaac
ccactgtccagccctggccagaagcatttctgggaactgctcccagccctactcccattg
ccaggtgagccccctcgctcctgcacacacacaggtgaaagacaggtctgagtgaaggcc
aggcctgggactccaccaccactactgccccagacataggaatgtgtacgtgggtgcaca
ctcgcctgcgcacatgcctgagatccagatcattccttcaaagcttctttgcaggtgcaa
tgatgcagcctcactgctgccccagccctgagcacctacaaggcagctgactggtccttc
tgggtttgggtatatgggttggggagagtgaggcagggacacgtgcctgaggacttctca
ggtggatggtgtccaggagctgagcaggccatggggcttggttgtgggcagaatagagag
ttccagccctgggccagggggtgggaagagaaggggaggggggtctttaagagaagcaca
gtgagctctttctgatctggtataaacgaagcccttcttggagacagaaagatggtcaag
ggctgacaaaacccttccagtgcttaatgaatgtctctctttaaacctggccattggtgc
caagccccttaagttccatattcatagagctcagttataataagaataacagctaacagg
cttactatttgcaggactttatctatgactcaccctgcaggcccagcccccacccccagc
tctccttttccacaatggaactggaatagagttatcttcatagaggcctgttggaggagc
atctgaaatgcaagaattctggggagggagcctggaagtgtcctctgctcaggcccacaa
ggacagcagagaaatggaagggagtgggaaaccgtgtcattttcttaccagaagacttag
tcaagtgctcacaggtgtggtctgcaggcagacgtgggcagcaccagacaaggagagatg
gagggtgggccctgggtccaggggtgaggagcagtggagtaaggagaggcacagctgatc
ccctgcctgccaggagagctgccgctgctggctggcgggaaaggagtgtgatggatgctc
ccctgtccagctgcgcctcagccttggcagaggaaggaaggacgatgagcccctgagcca
gggaacacggatgtctgcctgaggaggcggagaccaaggcagagacctgctctcccactt
ctcctccccaccacccccctgcctcggcacagcctgggtcgacactgagcactcacacca
caaaacatgcagccctgggctcaaatcccagcccaggctcctttagcctctctgagtcag
ttctctcaaccgtaaaaccacacagggcagttgtgaagattaaaagataatgtttgcgga
agcacatagcactgtctggcacctagaaaacactcaataaatgtcagtcactattttatc
atcattattagtgttgtgattatgcagaccttccatctctctctccagacgctcacaata
gcctcctaagtacgcttcctatctttcatctctcctctccaatccatcctacacaaagct
gccagaacacagtctacagcaatgatttggtcacattactctgctgcttaaaacccccac
cagctccccactgcctacacaaccaaatccaaacaccctgcatagcaaggactctccatc
tgcctcagactccctctcctgccccctctctggccattccccctccatccaacctcctca
acacatcccaaatctagaaactcttcatgtcatgcacttgcccacctcgatcccttcgct
tctgcttgcccgtccctgtagaatgccctctcccaaatgccagctcatctatgaagctcc
ttctgaccctcacgcagccaatggcttcctctgctgacagatgcagcatgtggtttatac
actctcatcacttcctcaccctgtgttgggctgtgcagatgcaagaagtgcttctcaaaa
tgtgctcctaggaccagcagcatctgcacctcctgggaacttgtttaaaatgcaaattct
caggcctcaccccagacctactagatcagaaagtctgggggcagggcccagtaatctgtg
ttttaaccagccctccagactctattgcacactcagttggggaaccactgatgtaaggta
cctgtaccctacaggtgatgcttggtacacagagagaaatgatgggttAGATCAGACCAC
TTGGGTGGct
Primer
(+)CAGCCTTGAGAAGGGAGATG
(+)caCCACCCAAGTGGTCTGATCT


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