Human element [ hs1889 ]Position: chr3:122,690,302-122,695,291 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: SEMA5B(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CAGCCTTGAGAAGGGAGATGtggcaggtgataattctgcttcaggtaattgatcggaaat gttttccaaatccatcatggggcatgaagcaaagatattaacaggtctgccaagagccag caagaataaggtcttatttacctctggatcctcagcccgtgtctattgaatgaatgaaag tgagtctctcctcctgaatgcccagtggggaaggtgttcacacacttattgctgcatggg tgctggtgggcctctgcccaaggagccttccccaaggctttctggaaggcaggtgcggca cggtgagaagagcatggacttggaatgggaagccatggtgcaagccctgatgtcagccac tcctgaactcgtcaatagacatgattccagtgggagcaacaggcaccgtgttctgcatcc ttgggccccgctgcctcccttctctttgaaacagagagaataatgatagcaacaacaaca gcaatcataacaacttcttcacagacttgtgaaggtgagatacttgtgcactgagagcgg aacaagcactcaacaaacgcttgttgtccacatggatccgctgatcgatccaccatggct gggtcacttctccagtgtggagagttttgaggagtccctcactgctccaggtaggaagct ctgccactacctgacttacttttcctcttgctgcaacttaaaccctcttactccactgtc tgtggtgacaaggaataggccgacagttggtgaaagctctctctaacactggtctcccct gcttttccaggaaagtagcagaaacagactttttggagtcaggaactaatgttatggaga gtgtgcagtgacttaagatgtatctaaatcagtgcaactccaagccacatggtaacacaa atggttctgctgcccatgtataaagcatgccagcccttttccctggcatgtggcacactc cagatctctcccctcatccagttatgtgtgtgcaaaactcagcctggatcagtcaccctt gaaatatgagtcaaataaaattaaaattaaaaattgttcttgaatcacaccctgttttgc attatctgtgctattatttgactttactcagccctttaaccactggctccagatgatccc acagatgtaactgggccagcactggggagagagacccccacctgagtcggtaagagctgt tgggatgctgcgctgggtgcagcataggtgaccagcagaaaggtagtgtcaggaatcatg caaatccccttcatgtggatgggtccttgctactcttcacaaccacgatgccatcagatt ccctgtgaagtctgcagaaggcttgtcatggctcccatttaactgatgagaagccagagg ccctgagagcagctacagatccaaatgctcagagtgggaagttagcccaggtctgccttc ccaccccgcagcctatccaggctcccagaaacactcttattccccaagtcaagccgtgat ggctcacactggataaggtccccagccactgggagctgggagcctggaaactgtgggaga acccctacagattaatgacaggtcattcagagtgtctatacaccttgttttggaaatcta agaggtaaccatagttagatcccaaggtctccagaaaaatcactcataatactctctttg gggagtgggcagtattgagactggggcaggaaagagaaatgatggactatcatcccagtc tgagcccctgactccagcctgtgttcaggctatgtttatgtccttctctccctttacacc cctagcttctagtctaggtgccaagtggccctgggcctctagtgccctgaggagaggcag ccaatgagctgctaacccagaggacataagctagtccaactcagcctctgccatgtgacc agccaaaagcagaacctcaaacccccaccccaagatgagtggggtccagcctggcgggca gtggagggtgccgcagtctgctccctttcctcttttagagaggaaaaaagaaaatgagtg aggaggggtggaacgtcaacagagaccagaagggaataaagaacactgaggccagttcat ctgtgaaggtcgtgaaatggacagagttttgttccttttggtttatgttatcgcagttat gatggtgtttgtgcagtaagtgaaacagtgaagggaaagtgtctggggtctgtgttggag ctgaacgatgctgcaaacttggggggttgtagattcccaaggagggcaggcaatgtggag cctccctgtcccactctgggaatctgcaacccaccaagtgtggagcccaggacagagggg cagaggtatgggagtggggaagggcagagagggaggagaaggtagggagaaattccttaa accctttcaggatttccttctggagggctgtgagtcttcttcaccaggcccaatacctgg tactctgggtcctgccagctcagatgcctgaacttccagaacataattgtgttccagctc agcagggcacagcctcgggccgtctaggagcaggctgccgttccccgaatgtacaccctg tcagtgcccagatccatctggagagcaaatggctggctgaggggagagagagcccaggca caccaccccccatctgctcccgccatgtgacacatgcttgcaggcggggagggagaagcc ggcaggtggttggctgatgtagagtggaggctgggagaaagaacaaggaccagcacaaac ccactgtccagccctggccagaagcatttctgggaactgctcccagccctactcccattg ccaggtgagccccctcgctcctgcacacacacaggtgaaagacaggtctgagtgaaggcc aggcctgggactccaccaccactactgccccagacataggaatgtgtacgtgggtgcaca ctcgcctgcgcacatgcctgagatccagatcattccttcaaagcttctttgcaggtgcaa tgatgcagcctcactgctgccccagccctgagcacctacaaggcagctgactggtccttc tgggtttgggtatatgggttggggagagtgaggcagggacacgtgcctgaggacttctca ggtggatggtgtccaggagctgagcaggccatggggcttggttgtgggcagaatagagag ttccagccctgggccagggggtgggaagagaaggggaggggggtctttaagagaagcaca gtgagctctttctgatctggtataaacgaagcccttcttggagacagaaagatggtcaag ggctgacaaaacccttccagtgcttaatgaatgtctctctttaaacctggccattggtgc caagccccttaagttccatattcatagagctcagttataataagaataacagctaacagg cttactatttgcaggactttatctatgactcaccctgcaggcccagcccccacccccagc tctccttttccacaatggaactggaatagagttatcttcatagaggcctgttggaggagc atctgaaatgcaagaattctggggagggagcctggaagtgtcctctgctcaggcccacaa ggacagcagagaaatggaagggagtgggaaaccgtgtcattttcttaccagaagacttag tcaagtgctcacaggtgtggtctgcaggcagacgtgggcagcaccagacaaggagagatg gagggtgggccctgggtccaggggtgaggagcagtggagtaaggagaggcacagctgatc ccctgcctgccaggagagctgccgctgctggctggcgggaaaggagtgtgatggatgctc ccctgtccagctgcgcctcagccttggcagaggaaggaaggacgatgagcccctgagcca gggaacacggatgtctgcctgaggaggcggagaccaaggcagagacctgctctcccactt ctcctccccaccacccccctgcctcggcacagcctgggtcgacactgagcactcacacca caaaacatgcagccctgggctcaaatcccagcccaggctcctttagcctctctgagtcag ttctctcaaccgtaaaaccacacagggcagttgtgaagattaaaagataatgtttgcgga agcacatagcactgtctggcacctagaaaacactcaataaatgtcagtcactattttatc atcattattagtgttgtgattatgcagaccttccatctctctctccagacgctcacaata gcctcctaagtacgcttcctatctttcatctctcctctccaatccatcctacacaaagct gccagaacacagtctacagcaatgatttggtcacattactctgctgcttaaaacccccac cagctccccactgcctacacaaccaaatccaaacaccctgcatagcaaggactctccatc tgcctcagactccctctcctgccccctctctggccattccccctccatccaacctcctca acacatcccaaatctagaaactcttcatgtcatgcacttgcccacctcgatcccttcgct tctgcttgcccgtccctgtagaatgccctctcccaaatgccagctcatctatgaagctcc ttctgaccctcacgcagccaatggcttcctctgctgacagatgcagcatgtggtttatac actctcatcacttcctcaccctgtgttgggctgtgcagatgcaagaagtgcttctcaaaa tgtgctcctaggaccagcagcatctgcacctcctgggaacttgtttaaaatgcaaattct caggcctcaccccagacctactagatcagaaagtctgggggcagggcccagtaatctgtg ttttaaccagccctccagactctattgcacactcagttggggaaccactgatgtaaggta cctgtaccctacaggtgatgcttggtacacagagagaaatgatgggttAGATCAGACCAC TTGGGTGGctPrimer
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