Mouse element [ mm1865 ]Position: chr13:15,240,889-15,246,348 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: AW209491 - Gli3 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence GCTCCAAAAATACAACCTCCAGatgctccaatctcctttcacagacacagatgcttctac tggacttgttccttctgatctatccacagacagttccacagaacctggacaacaaggaaa cagctaattctcctcagactagaccaacattcccatacctatttttctcagtttcctaga gaaatgtcaccacatagccagaaggaagtagttaaagatcacaatgatcctattcctgct tcaccatcacttctttctcattttttttcttttttaattaaaccaaacaggagaatgtta gcatttgatcgaagctctgccccacagttacctgggaacagccaggtatgttgcacccca cagttacctagcaatagccagatatgcatggcttactataaaaggtactgtttgttccct cctctctctctagctgttgttcttgccttcctgctctgtcatctcaccctttcccccctc tctcattccctcccccccccacatgctcatggccatggtctctacttctcttctcttctc ttctcttctcttctcttctcttctcttctcttctcttcttcttcttcttcttcttcttct tcttcttcttcttcttcttcttcttcttcttcttcttcctctctctctctctctctctct ctctctctctctctctgcctctactaccctcgtaactcccctccccataccctaaataaa ctctattctatagtataataccatgtggttggtccctagggggaagagatgcctcagcat ggacaatggaggtatctccttccctcatacctgactatacttccaccaaaacatatttct cctctcttttttataaacacatcactatgccctatatgatcggacatcttctttctgccc caaacttctccgtctttatcagacacagaaattacaaaatctctacatatacatatgcca ctacaaaaatacaaacagtatgaaatatcaaggtagtatctctcttcaaaatgtcaccag tctaatagagctcttttctaatgataattacatatatgagcccaggacaaaaaatttaaa agaacaataataaaaaagaattcaaggagtctaaagaagacacaaagaaacagcttaacg aagttatggagaaagagcttaattacaataattataataagataataataataataacaa gagattaaggagaataattttatccaagaaaacacaaacataaggctgatggaaattata agaacaatccagaagttgagaacaaaaatcagtaaaaagataaaaacattgaaaagaact caagctaaaatgaaatggaatttaaaaccccagtaatccaaccagaaaattcaaaggaaa gccttacaggtagaatgaataaaggagaagataaaatatcaagagttgaatataatacat aagaggaaaataaatgaaagtaaaataaaatcaaaaagataggcactgcactagagtgaa agaatgtttaaaagaattaaaatcaaattggacaaggaagaaaacaagagttgctatcta atatctgccagaatagttcaacctaaaactaatcagaaaagataaagaagattcttcatt ctaataaaaaaaaagatgaccaagaaaacattatcataaacatatatgcatcaaaatata ttgcacctaatttcataaaaatatactatttgagttaaagacacagatttggccatgtgt gttggtatatgcatttaatcacaacaatcagagggcagactttaatgagcttgaggtcag ccttttctacacagtgaattccaagacagccagggctacataaagaaaacctgtctcaga aaacacacacaaataaatgaatgagtacatacataaatacacatattaacatcaactcat aaatattagatgatttttaattcactattttctccagtagacagatcatcatagtagaaa ataagcagataaacatcagaattaaacgatgtcctatatcaaatatactaaaaagatatc tataggacattccatccaaacagcaaggaatatatatcctattcatcaacaaataatcta atgggaaactaggaaaaaaggggagttatctaattagggaaaaaacaggaagatgaatac aaaaaaggacagagaagactaaaacagcagtaaggtctaaaaaaaattattaggaattgt aatattaattaacctaaaccttttacagaaattattaattaacctaattatttacctaag aatccataatataaataaatctgtatatgtaaatatgcatacatagttcaaatgaaaatt tcttaactgatctagtatcacctaacaacaacaacagaaacaacaagcataagaaaccct ttcttaatgaggtacatttacacaatggagtactattcagctattaaaaagaatgaattt atgaaattcctaggcaaatggatgtacctgaagagcatcatcctgagtgaggtaacccaa tcacaaaagaactcacatgatatgtactcactgataagtggatattagcctagaaactta gaatacccaagatacaagatacaatttgcaaaacacatgaaactcaagaatgaagaccaa agtgtggacactttgccccttcttagaattgggaacaaaacacccatggaaggagttaca gagacaaagtttggagctgagacaaaaggatggaccatctagagactgccatacctgggg atccatcccataatcagcctccaaacgctgacaccattgcatacactagcaagattttgc tgaaaggacccagatatagctgtcctttgtgaggctatgccggggcctagcaaacacaga agtggatgctcacagtcagcttttggatggatcacagggcccccaatggagaaactagag aaagtacccaaggagctaaaggggtctgcaaccctataggtggaacaacaatatgaacta accagtaccccgccccagagctcgtgtctctagctgcatatgtatcagaagatggtctag tcggccatcattggaaagagaggctccttggtcttgcaaactttatatgcctctcagtac aggggaacaccagggccaagaagtgggagtgggtggggaggggagggggaagggtacagg agacttttggtatagcattggaaatgtaaatgaagaaaatacctaataaaaataaagaag agtttaaaaaaaaaacccaaaaccctttcttttgagttattggtcagtgttgtccaaaag actgttaaaatattacattcttttgatattgcccttggttactctcttccaaccccaaga aatgaaacctaagtccctaattgctgaagacactgtgcattttagacacagggcctagag gaccttcagctaaagcttaccttaaagcttcctccctaaggattagctttaattgttaca gaagattctgctcaagattccaaaggaatgaaacaaacaacagcccaatgtagctatgac acatgagtgacaacaaagactagcatgccacaataacctaagcactgcaatactggcatg tgtatcttggtgataaccaaccactcttatgtataaggtatgttcaacaagagggaaatt ctgcttcatactgaaaaattagccagctgtctagcccacttcatactggaaaccagggtc agtaaagtcatggatcttagaagaaagcttacagttgttactcttctaaaccatcatagt tcctaaatacattttaaaatacattgtatttgtccttatacccacaggctagtatagttc ttatccttcatcaagaaaacttctctgcaaatggactaagaccattacaaaaagctacaa gtaatcaaaatacaagcatgtgagttcagtcccagctgatacatctacaacacaatcctg tacccgctcaaggatcaatggaaaaggggctggaggaaattgtaaaaggcagagttttct gtgaggttgtgtctcctaaaaatgtcagagaagcaacctacccctaagaaatctcatggc ttcgtaaaataacctgaacaaatataacaaatgttaacatggaatggggaaggctcagaa gatctgcaaccctaaacaaagaactgtaagcaactaaggaaggctgagagcagaagtagc cttccccaggaaagaacacactagtcggctgccagtgccaaggtaacccctgaaaacatg tgcatgcaagaaaaattatatggaataagcaggttataattatatatttaagaatacaca gacatacatgcacatacacgtatgtaacagcaattaaagagggagggaaagagcatggat gagagaagatgaggtagagatctgtgagaggttttggagggagaaaagtgaaggggagaa agatacaactagtatcttaaaaaagtaaatacacatattaagaagaaaaaagatcaaatt cttaactgagaaaaatatataaataactaatgggcacagttgaaagctttcaaaatcatg gtaatcaaagaaaatacagaaatctgtcatagatgagaactgcctacatagaaatcattg ctaaatacattgtgatattctggaacacaagattatagacagaaactaaggactcagtac tggaggcatgtaccagcagttaaaagaacaaaactgattttgcagaagatctgaatcagt ttcctgtgtcagttgcagcgcaatcgtctataacaccagctccagggctctgatgcctct ggtccccatggacacacacactcatgtgtgtgcttacatgcgtgcgtacatatgtgtgct cacacgtgcatgcacatgtctggactcacacaaacatgtaaataccaaaattgtttaaaa ggaaacacaactaaggctttgggctggagaaatggcacagcattcacactgggtggctca cacccacctgaatcttcatctgcatgatgcgtctggtacctgcatccatattgacatgct cacacacaggcaccttcattcacaggcgcatgctctaacatacatacatataactgatgt taaaacttttaagctaaggttatgaataaaatatggatttgggctaataatactttatcg gtatttgttcattaaatggagtgaatatacaacaagagtaaaatatttataaatggagtg aacatacaacaagagtaaaatatttatgagtaactgcacataggcacataaaagtttctg tatctttacttttttggtaactataaactaccaatttcattagggaaatattcatatact aacttagaagcaagaaaaggtcatttctatgggagagaaacaaagaggaaatctatgata gttggcaagatcagaagatgaggggctaaatatggtgcCAGACCCAGACACAAGTCTGAGPrimer
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