Mouse element [ mm1861 ]Position: chr12:65,247,302-65,258,785 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Lrfn5 - Fscb Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence tAtgTCTTACACGCGTTCTCACgaccggccaggaagaacacaacaaaccagaatcttctg cagcaaaactttattgcttacatcttcaggagcaagaatgcaagccccaacccccccatt gcttacatctttaggagccagagcgccagagcgccagagcgccagagcgagagctctatt gtttacatctttaggagcaagagagcaagcccaaagcccaaaaacgaaaacgaaaccccc tccctcttaaggagagttatccttcgcctaggacgtatcactccctgattggctgcagcc catggccgagttgtggtcacggggaaggcagagtacatggggtggagaactacctttggc acatgcgcagattatttgtttaccacttagaacacagctgtcagcgccatcttgtaacgg cgaatgtgggggcggcttcccacagttcccccttttctattaataaaagcaataggccac ccatattaatgagagtggagatagaggtcaaatccccagtgtgcaggtaaaggagcctgt attagtcagggttctctagagtcacagaacttatggataatctctaaatagtaaaggaat ttattgatgacttacagtcggcagccgaattcccaacaattgttcagtcgcagctgtgaa tggaagtccaaggatctagcagttactcagtctcacgcagcaagcaggcaaaggagcaag agcaagagctagactcccttcttccaatgtccttatattgtctccagcaaaaggtgtagc ccagattaaaggtgtgttccaccacacctttaatcccagatgaaaggcatagcccagatt aaaggtgtgttccttaactcggagattcaatcttctggaatccatagccactatggctca agatcttcaaaccaagatccagataaggatctccaagcctccagataagggtcactggtg agccttccaattccggattgtagttcattccaaatattgtcaagttgacaaccaggaata gccactacaatccaccccttgtcaacttgacacaaataatatctcatgttcacatgaaac aataacaaggttgtaaatacgcctaacatgatataactatccctcgtacaatcgcaaacg cattagtaaatttacaatgggcattcatattactttataatcctcgtttctgcaactggt tacgtggccttaattggtatttataactaccttcctctactacccattctgtatttcctt caccttcagccagcacctcagcaggtcttggctcttttcctggaggattgacccatacct tcattcctgatgggtctgcgtcctttgtcatcctgcttggattaggctgttgtagtttcc cattgactttaatcacaggacatggtagtactaagagacgccctaagggatctcctgcac tccagacataatcttgcttaccaccattgtgaagaggtaatccaatttccccatggtaat ctggatctatcacccctcctaacactgttattccttttttagcctgttggtttaagggca ttagaagcccaaaatgaccagggggaagtctgagcttccagttcaatgaaatgtttgttg tagctcctggtaggagcactcccctctctggagccaaaacttctaagccagcagaaccta gagttatggggacaggaagcaaaaattttcctagagggtcactaggagtgatagtaagtg gaactattccgttttccaccccttgattcctggacccatgaatcctggctatgggtgaaa ctgtaccatatatcgagcgctgattcaaagcatatactgccttctgaagaactctgcccc agccttccaagctgttaccacctaattggcgctgtaactgcgtcttcaaaaggccattcc atctttctatcagcccagctgcttcaggatgatggggaatgtggtaagaccagtgaattc catgatcgtgagcccactgtcgtacttctctggctgtgaaatgagttccttggtcagaag caatactgtgtggaataccatgacgatagatgaggcattctgtcagtccgtgaatggtgg ttttagcagaggcattacgtgcaggaaaggcaaatccataaccagaataagtatctactc cagtaagaacaaaacgctgtcctttccacgaaggaagtggtccaatgtagtcaacctgcc accaggttgctggctggtcacctcgaggaatggtgccatatctggggctcagtgttggtt tctgctgttggcagatctggcaatcagcagcagctgtagccaggtcagccttggtgagtg gaagcccatgttgctgagcccaagcataacctccatctcgaccaccatggccactttgtt catgtgcccattgagcaatgacagggatggctggggagagaggctgattgtccacagaac gggtcatcttatccacttgattattgaactcctcctcagctgaagtcaccttttggtgag catttacatgggacacaaatatcttcacatcctttgcccatttggagagatctatccaca tacttcttccccagatgtctttctcaccaattttccaattgtgatctttccaagtccctg accatccagccaatccattggctacagcccatgagtcagtgtataatcgtacatctggcc atttcttcttgcaaacaaactgtaataccatgtgtactgcccgaagttctgcccactgtg aagatttcccttcacctgtgtctttcaaggttgtcccagaaaggggttgtaatgctgcag ctgtccacttctgggtggtgcctgcataacgtgcagagccatcagtaaaccaggctctag tcttctcctcttcggtcagttgatcatagggaacaccccatgaggctataggtgcatgct tggcagcagatggcattgtaacaggagtagaaaccataggcatttgagcaacttcttcat gtaacttgcttgtgccttcaggacctgctctggcccgatcacgtatataccacttccatt tgataatagactgctgctgtgcgcgtcccactttatgacttgcagggtctgatagtaccc agctcatgatgggtagttcaggtcgcatagtaacttggtgtcctattgtcaaacgttcag tttccactaaggcccaatagcaggccaagagctgtttttcaaagggagaatagttgtctg cagatgatggtagagctttgctccaaaatcccaaaggtcttttctgtgattcacctacag gggcctgccagaggctccaaacagcatctctatcagccacagacacctcaagtaccatcg ggtctgctgggtcatatggtccaagtggtagagcagcctgcacagcagcctggacctgtt gaagggccttctcctgttccaggccccacacaaagctagcagctttccgagtcacttggt aaataggcctaagtaacacacccaagtgagggatgtgttgtctccagaatccaaatagac ccactaaacgttgtgcttctttcttggttgtaggaggggccaggtgcaataacttatctt ttaccttagaaggaatatctctgcatgccccacaccactggactcctaagaatttcactg aagtagatggtccttgaatttttggttggatttatttcccatcctctgatacgcatatgt gttaccaatgagtccaaagtggttgctacttcctgctcacttagtccaatcagcataatg tcatcaatatagtgcaccaatgtgatattttgtggaagatccaaacgatcaagatccctt ctaactaaattatgacacagggcaggagagttaatatatccttgaggcaaaactgtgaag gtatactgttggccttgccaactgaaagcaaattgcttctggtggtccttatggacaggt actgagaagaaggcatttgccagatcaatagccgcataccaggtgccaggagatgtgtta atttgctcaagtaaggaaactacatctggtacagcagctgcaattggagttactacctga tttagttttcggtaatcaactgtcattctccatgatccatctgttttctgcactggccag ataggagagttaaatggagatgtggtgggaaccaccacccctgcatctttcaagtccttg atagtggcagtaatttctgcaattcctccaggaatacgatactgtttttgattcactatt ttctttggcagaggcaactctaaaggcttccatttggcctttccaaccataatagccctc actctacagttcagggaaccaatatgagaattctgccaatttctgagtatatctatccca attatacattctggaactggggaaatcaccacaggatgtgtccggggacctactggacct actgtgagtcggacatcagtcaaaactccattaatcacctgccctccataagcccctact ttaactggagggccacaatgtttcttgggatcccctgggatcagtgtcaactcagaacca gtatccagcagaccccgaaaagtctgattatttccttttccccagtgtacagttaccctt gtaaaaggccgtaggtccctctggggaagaactggagaaagggtaacagcaaaacctttg agtgtcttatcaagatccttcctcagaggaacctggccaccccttcattcaaggggttcc ggatctgcaaactgtctcaagtctggaaattgattcactggccgagattgctgtttacca cgatctaatgtagcctttctttcatttgtttgagaatttttctgcttatacagatcaaac aaatatgcagtaggcttcctatgtatttcattcctggaaacaccatgattgattagccag taccaaaggtccaagcgagtcatgccattataaatttcacctctcctctgctgaccatta ctgggtatgttattataaacattcttttgtctacgctgtccattataataactaggatca ccttgtctccggcgattcaatgctgccacctggcccttgttacctcgggatccaactaaa cccagtgaatttaattcatctaattgagcagaagcatctccaatgctaagatctggcaca aggaaaagggaaagaacaaaacccttcaaatgtgctggtgcccctctcaccaatttgcgt cttatagagctggtgaaaggcatatcttctggaccttcccattgtggacaattatgcttt acacaatatatccactctagcattgcaatttccctaagtcttaaaatcccttcatcaaca ctaagccacggaatatcaggcatctccaagtcatttccagtaggccatcttttgataaac acctcagccaaccattcaaacaaacttttgacaccttttttaactatgcgagcttccgta ttaaacctagaatctctactcagaggacccatgtcaataaactcagcctgctctagtttt atgttccttccacccttatcccacacccttaaaatccattcccacacatattcaccaggt ttctgcttgaatgaattagcaaactcattaagctccttagtagtgtagcgaatttcctca tggactatactttctacctcccctctaggagcctgttttgctttgagtctggttacaggt ctagaagaaactattggtgggccgtgagcagactctgcaaaattaatttcctcatgtggg gaaggcattatttcaagaggtggggctgagggtactacttcctcaggtggggcaaaccct tgagaatctgaggattcaaaattctcagcttcaacatggtcttcccacacatccccgtcc catgttgtaggatcccattctttgccaattagagcccttactttaactgtcgacacactc tgaggctgagacttgaattttctctgtagttcagccaaccttacaatgagagtttctgtt tgattttctgcaacttgagctctattgctacaagagagaagattctcctcaaggacacac ttagcaacttttagatcgtttacttgtgtctggagcctttcgattttatcacacaactcc ttcctttcattcatcattttttccacagatactaagagcagccagccagtaaaatcattt ttcgattttttccccatcttgtagaaagctttgtgcactgaatcacctaattcattaaga aaatcaagggcattagcttctttaagttcggaatatagtttcaaccatgggtcttcaaaa ttctctgagctcccaggagggagagaatctggagaggtttcaatatttgaaagtgctggt ggatcaacaagccaattccagtattttaaaagattcatcctgtacttctgttactctaga accactcctggtaccaacttctgtattagtcagggttctctagagtcacagaacttatgg ataatctctaaatagtaaaggaatttattgatgacttacagtcggcagccgaattcccaa caattgttcagtcgcagctgtgaatggaagtccaaggatctagcagttactcagtctcac gcagcaagcaggcaaaggagcaagagcaagagctagactcccttcttccaatgtccttat attgtctccagcaaaaggtgtagcccagattaaaggtgtgttccaccacacctttaatcc cagatgaaaggcatagcccagattaaaggtgtgttccttaactcggagattcaatcttct ggaatccatagccactatggctcaagatcttcaaaccaagatccagataaggatctccaa gcctccagataagggtcactggtgagccttccaattccggattgtagttcattccaaata ttgtcaagttgacaaccaggaatagccactacagagccgtacacataacctccttccagg ctcatcacctagaggggtcctggtctggtcccgtgttgtttttcctggaggaaggacact tgaacactcaaccttcttgaaagatgacatgtctccctagaataggctcatatatgccgc agagcccttctactgcagtgcttagccgtgcaactctctcgggctgctgaagcacactca ctctgtcccgtgcaatgagactagcctcgtggtgtgcaagagctgagtggccagcgacct attgcttaagcatagataaccataaatcaggggaagcaccatgttctagtcctgcaagcg cctgggcaataaccaccttgtctctcctagtttgggccttaagcttacagaccaaccaga gaagcaacactaatccacagcaaagtgtatctccaaataatatcaatcccacccattctt taaagaaggaaaatgctgaggagatccaattgggtaatcctttggtcagggacaggtcta agcgcgtggagttgacctgaagtctcaattcccgaaggatctgttcaaattcagccgtcc aattctgtaacatatactgagaaagactttttgacaaattagctgccctagtaaatttct catactgaatggaagtaacacacaatcccggaaacttttgttcacatcccagctgagcta tttgccataatacatctagttgtttctggacaagatctatgagttgattaaccagcatga gaccaccctgtatcttgacattagctgaggcctgtttatctatgactgtagtcactgagg ctgacaaagtgttaatggtgtcagtcgtctggacctgtccagacagagccaaggctgtct gaatcaaggccaaacccagttcctagttagtggtaaaaaagcaggggaatacttgagcat tatacattaccggcattgggagtggaaatgtcgacattatcccccaacgctgctctctct ttattattgacgccctggacatcaccaagacgagggacatcagtattcccttggtcagtc tggatttttcgggtgagtctttctggtatccaaaatgggttgtcttcattctgtgggaaa acacaaatagctcccctggatcttatcaaaataggatccgggccataccatttattatca aggacatttttccatttaaccatctcattgggcctatctggctctgaacaatgacgttca gccgcagtatggccatgagcatcaatatttaaaaaaattgagtgtaaagagtgccataga cacagacactcttggtactcggggtagagtctcgactcccctcttctgttttaatagata ggatttgagggtgcgaggcgcacgctcaacaataccctgtccttgagggttgtatggaag tccagtcaggtgggtcacgtccatctgacggcagaactgctggaatttttgagatgtata agctggtccattatcagtcttaaggagtttgggtttcccccaagcactccatgcctcaag acaatgttgaatcacatgtgaggttttttcacctgttaaaggtgtggcaaacattacacc agagcaggtgtcaatagaaacatgtaaatattgatttctcccaaaggaggaaatatgcgt gacatccatttgtcagacctgtaatggcctggcatgagtagataaaatttgacaacaatc taaaggcattattaagtaatcagaatcactttcagtagaaccaatccctctggcagctca aggaataccagaggaaggaaaacagccatgtaggcttggcaaaattattagttgagctat tttgtccctttgtgatatagaaaagacaacttgaggacaagaacagagaacctgaagttc ccctttataatcctgatctacaactccagggtggacaataagaccttgtaggctgcaaga gcctgcctctgtcatactggccacaaaatctgagaaggactcctaaggtccctggacgat ctttgttagttgtccagtggcttcacctgctcgagagagcgccttccaggccctaatagc cttttcatctgattcagaactactaagaactggctccttgagctcacctagtgatgagta tctcctagagacctccgctaattgatcttttttcttttcttttttcttttccttccttct aatctctccccaggtattcttacctgaccttaacttttcctcgggttcaagacccttgga aaggcctgtatacttattttgtgtaccatatttcctctttgctcctactctctctccccg ctttacttctgatagattgccctgaatttcatccagaattttcagccctaccttaaccac ttgataacatgtgaaaaggaacaaaaaggctcctaacattagaaaaagttcaaggccaaa cataccttgtaaagctatttcccactttacttctgatagactgtcttgaatttcgttaga aagttcaagaccagacttaccttgtaaagctatttcccactttacttctgatagactgtc ttgaatttcgttagaaagttcaaggccagacttaccttgtaaagctatacttacgggtac cctgttccccagctgaagagttctgaattcacgcagttgaatccttctcaacagtctgtt ttacgggaacacttcattaccaccattccccagctgaagagttctgaatccacgctggat ccttctcaacagtctgttttacgggaacctttatgaccgtgacccgcagttctggttccg gaatgaggaatcttccttgcgccggtgatggttaactcccgtcccgagttttcttgtccc gggtttcgttcttcccgggttttcggcaccaactcttacacgcgttctcacgaccggcca ggaagaacacaacaaaccagaatcttctgcagcaaaactttattgcttacatcttcagga gcaagaatgcaagccccaaaccccccattgcttacatctttaggagccagagcgccagag cgccagagcgccagagcgccagagcgagagctctattgtttacatctttacgagcaagag agcaagcccaaagcccaaaaacgaaagcgaaaccccctccctcttaaggagagttatcct tcgcctaggacgtatcactccctgattggctgcagcccatggccgagttgtggtcacggg gaaggcagagtacatggggtggagaactacctttggcacatgcgcagattatttgtttac cacttagaacacagctgtcagcgccatcttgtaacggcgaatgtgggggcggcttcccac acttatgggaggggggggcaatagaaattctttcttttgacagctacacggggaagcagg aacttggtggtgtcagggtgtggtcaggagagcggtgatgacccagggctggaacatccc gtagttctcggaatccatgtgtcggtggcccgcttttgaccccttttttttcttctgggg ggggagaggcccaattcagagatcaggacaatagcgttacctaaatggctcccaacagag tgaaacttgtttgttaggtaccccccaaatagattttaatctctcacatttttcccttta cacaattggtatatgatacacaggtatttttttgttttctggtagcatttcattaacact tcaaatgattaaatatgagttttgttttagaaaggtagtgttttacatgatacaaatctg accatttcctattcttctatctgattagcttgatagaaaaaaggaaagtaaacaattatt tttcttttattattttgaagtttatcagtttattttctatgtatgtcttcaattttattg gatattttatttacaattcaaatgtaatctcctttccaagtttccactttataaaccccc tatatcattccaacttactctgcttctaagagggtgattccctacccatccacccactcc tgtctccccacactggcattaccctacactggggcatcaagtcctcacaaaaccaagggt ctgtcctcccactgatgcccaacaaggccattatcgtcaatgtatgcagctggagccatg ggtccctccatgtgtactttttggctggtggtttagtccctgagagccctggagagtctg gttggtggatattgttgctcttcctatgtggttgcaaaccccttcagctccttcagtcct ttctccaacttctccattgggcactctatgctcagtctaatggtccactgcaagcatcta cctctgtatttttcaggctctggcagagcctctcaggagacagttatatcaggcttctgt cagcaagcacttgttggcatgcacaatagtgtctgggtttggtgtctgtatatgggatgg atccccaggttgagcaatctcttgatggcttttcctttagtctctgctccacacttttac tccatattttctctgatgagaattttgttctcccttctaagaaggactgaaggaactaca ctTTGGTCTTCCTTCTTCTTGAGgPrimer
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