Human element [ hs1719 ]Position: chr8:32,063,683-32,067,187 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: NRG1-IT1 - NRG1-IT3 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence aAgaTGATGGAAAAGCACCTTGTGatttatctcttttggtagcagagagctcatgatcgt ccatctaactcccacaccatcttggagagccacagggtatcctacattatcctgctgaag atccttcagtacctaatggctctcgtgaagcattccaaagtgaaagtcatggagcctatg ccactaaatcagatataactagatacatgttggattaaaaggaaaggaggaaaggcatag aagggaaaaacagagattattggagaccaaatacctctataaattctcaaaatttgttta aaggctttactttttcaagatgcatattttggcaatagcttacgaagactgaagttttct tatttaatcttagagatactaatacttagaaaagaaaagtcttctatcttgttgaccttc tctgtagccatttgaaatcttcattatcagaataatgcccctccatgtccacaattatta atttttatagatttagggggtacaagtgcagtttcattacatggatgtattgtgtaatgg tgaagtctcggctttttgtgtagccatcacctgaatatagtgtacattgtacgcattaag taattcctcatccctttcccacaacctccacccttctgagtctccagtgtctataattcc actttctgtgtccacgtgtacacgttatttagcagccacttataagtgagaacatgcagt atttgactttctgtttctgagttattcatgaccagaattattattctaattattgtgaaa taaaaattatgcctgactaaaatgactgaatccagaacttcaccataatccatctatctc ctccgtgagtcagtgcatattacctattccaatgacctcttcagtactgtaaatattact gtgttccttttatttacttaataaatagatgtcaaatgtgtggaaaacaacatttgtttt ttcaaatacatgtttataacaccctttggaggaagtaagcctctagaacatgacagtctc tggggtatgctattttcaggtaattttaaatgtcgctttccaacaaaaatgcaatactat tatctttttttttaatgacaaagaaaagcaaaacaagtattatattggtgcaaaagtaat gcagtttctgccatttctcttaatgccattactcgtaagggcagaaatgaccattacttt tgcatcaacctaatacctccagattttagatatttgagtgccattgcttgatatatcttt tgttttttcttctggaaattttggttactttagttgcataaaggatgacttccattttac cctgttaagcatctctgtctggtcagaagctactactctgttctttctagttcagtaggg aacaatcagtttccttctcaattttcagctaatctttatgaatgaaatttgattaaatct agcaaaactatgcaaacatcataacagcatatggggagccttcctgtagtaccagtgggc tatttccgtctaataacagattcaaaaaatccaattagacagtttgaaaaaagaaagcca gagttaaaaatatctgactgtttgactgagaacagcagggaatggtttataggccaatca tttatggtgaataggatcttagtggtgagcaactcacatctctagcccattagaataatg tgttactcatattgctcctaaagctttctacttaattataccaagttaaatagcagacat tctcactcccttttgatggaaaatcacatttattcacccacacaataccatccttgggtg ttaagggatgagagttgacctaatgggaagacaaacaaatggtttaggaaatgagacatg atttttttacacaaaggttaatgcacgagaaaagaaatgcctaggtctctgtctaatgtc tatgtgttctcatcgaataattcttaaatgaccctatttagtttgattttctaactacat tagaaatatatgaaacaattaagaaatcattttttacctgtcatatggcagatggactaa cattgatcagggcagtcgaggaggttgtaaggaaatgggtactctcaaacattgctggtg ggactatgaagtgttttaattttttttttttttttagacggaggtttgctcttgttgtcc aggctggagtgaaatggcgtgatctcggctcactgcatcctctgcctcagcctcccgagt agctggaattacaggcaccctccaccatgcccagctaatttttgtattttcagtagagac agggtttcaccatgttggccaggctggtcttaaactcctaacctcaggtgatccgcccac gtcggcctcccaaagtgctgggattacaggcgtgaaccaccgggcccagccaagtgtttt aatttttaaaaactatttgaccatatgaaaatatagatgttccattaaatatatatagcc taagaaatataatcagcaatgcagtaaaagattatgtatacggatgctcctgacagttgg atttacaatagggaaaagctggaaacaacctaaatatttgaaaaaagagaatggtaaata catcaaaaatacaataaatacagtgttaagtgaaaagtttgtgcaattttgacttgagaa atttttaatgtactatgactacttggaagaatatggatgtacctcaaaataagtaatgat ctatatgaaagactgtttttgataatttttgttttcttatacataccttccaatattgtc caaattttcaataataagcatgcatagcctaatatatcatgtagatgagtgcaaaatgaa cacagagattaagatcatgggcttttcaatgaaggtctgagtttgaatcctgtctctgcc atttactagcaatataatttcaggaaataacttaacatctctgctcttcagtttcctcat ctgaaatataaggataataatggtatctgtgccttaaggatgaccaaagttatagatatc ttaagaatgaattaaatgcgtttctctccatggactattttaacaatgaatgcctgacat agcaattatgttccataatcaccattatttatttataaaattatttagtattatttaata ctaacattctggttttataaaaccaaattttaaaaatagataagtaaatgaaaggacaag gaaaacctaatttcactgtttttctagctttttttattgctataacttttatatcataaa tccacccatttgaagtgtatagctccattttactatactgttttcatttataaaagcctt ttataaatttatacatacctttcagtgttgtttgaattttccatagcaagcattcatagc atagtatatcatgtagcagggtttaaaataagcatagaagagggtttcgccaaaagatta tttaagaaaatctactaaaagagggttcttctgaaaaataaaatgtatttgcttttatca aatgAGCAAACTTCATCCACTTGGAPrimer
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