Mouse element [ mm1578 ]Position: chr16:72,690,938-72,694,980 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Robo1(intragenic) Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence TGCATGTGTGGCAAAGTGTGtatgtggcaaggtgcttgcttgtgtgaagggcaaaggaca gctttggggagtcggttctctcctgacaactgggtgggtcctgagaatcaaactcagggt ttagtcttgaaggaaagtgtttttaccctctgacccaacttgctgggtctataaaaagca acttaaactgtatttatttcaactctgtatttaatgtttatacttcccatgagcatagaa cttataaataatcattcaataagtatgttgaaaatttattattatttctgtgtttgttct gtgtgatagcatgcctaactccttttgactttgacttatcaacgtatgatggaaattgac ctatatgccaaaatctgcttgagattcaatcatgcttatctgtgtatggaattgtggtag aaccatgttcagggtatatattaccaaataacaaagactgaaagggtgagttttaaggtg agaaataatctagtgtattaaataacaatacatattacttagctgggaagaaaaaccaat gttggcaggtaattcttttattcccttattcttctgtgagagctattgctgtcatgaatc actaaggaggcataaaaatgtttagccaacaaacacatgccttgcttttatgcccattgt ggactattattttgactttctctagtttctcttagggctctgctaaattatttattgtat ctagttgcattgcaaccacttggaaaacagtaatttgtatgttaaatttaaacaatggtc ccaccaaagtgtctgtttctttttttcaaggctacattatttttttttaaagtgaattag gggataatcgacaataaggattaatttattcaccagaaggtggacacggggcagctgttt ctgtttctatagcccctgaggctgctggaactcagagttacgtgctaaagcagacagggc cattttcttcctcaagaaaaagctttattcctctgttagaaagaattaatgtcttacttt tgaagctcggatccatttttttatatatgataaataatagatgttatgttcctcagaatt caaattctgtgaggtagaatcctgcagatttctttcccaaatgccagaaaactccacagc tacccttttgtcttctgtttcaatcatttttgtttgcgaaacagttacttgaaagttatc atgaaatataatggccagctttaaaacccagaaaaggaagagaaaagaataaaccagcaa ttacaagacaaaaggttttcgggttgggaattaattgagtgacagaaagaccgccccaaa ggatacagctgaacacaggccctttgcagtctaatgcatggagatacagtttttcatgtt gaaatccatcattgattatcttggacccagttatgtttcttgcttccagcaggtggggat aaaaggtctctggagataaacgcaagccagtgtgtcccataagagaaatgctagactgca ggcttacttgaggctccaggattacagtgtgaaccttacaggagaggatattaggtttgt ttgtttgttttcttgtttgtttttctgccttcacaggtgtttcagtttggactaagtata gacagcttccccaaattagatcagataggatagcttgctgcccgtgtagggcatacatac ttctcttcataaaaaggtgtttgttaaattcatagctctgtttagttgcaaggaaaaccc tgacaagagaaattacaaggaaaatttaaaacttctatcagtaaaatatgagaaacattg atagagaaccagacttccatttttcctctgggatgaaaataatagttgttcatttatata ttcacaaatttcccccaagatttatttccgtgtccttgatgctcaagaaggcagcatcct ttttatttaccagacaatgcaggcatacagagtgtgtgaacacaagtgtgtgaacacaag tgtgagcgattcctttttccttttaacttggttacagctgtatgcctttagtgatttaag tgggccagcactatagtttgtaatagattgtgttttaaggagccccacgaagacaaggac cagaaatggccaaaatatttttagtctcacgacttcttggaaaagcataacaacaacaac aacaacaacaacaacaacaacaacaacaacaacaatcccccttctccaaacatttatcca gagaaagcctagccccttaccttcagaggctacatgatcaccacaaaatgcaatttttct tgatttagcaaatcttatacttcattgccagtataataatgtgaggcgtaggagtcctct ccacctgtcccgacatcgtgttattatgtgctttgttgcggttatacaaggcagtctaac agtgctcaactttgcagtctgtatagagccaaggggttattttaaagagaattgttgtga tcatctttccttcttatgtcttcattctgcatttgtgactagaaactaagggcattttct ccctttctagaaacctcaggggcactttgtcatgttttgggggaagaggttaactctcgg agagactgtagtgttttattatcagtaaatgtttttactctccagtgaaaggttggtaaa taggcactgtttgtgaacacaaagagataataatggcccagactttcccccttcaaatgc ttttttaacctaggaaaatgcattggtgatctcagccccagtgaacaatagctggaatca caaaaccactactcttcaatctgcctgcccggcataattagaggctttgactcagggtaa gaccaagggatgtttagaataggggcaagagctattctcccttaacttcccattgtgaag ccaaggctccatatgttgaaaaaggctgggacttctgctgaacaaggagctgccttcggt acctgttgtgcccgtgagaatgtttccccatgacccgtttggaggagactccattaacag tctaagggcaccagacttgtgagaaggaagcattgtctggccgtgcagtaaggtcacata gtgctgaaccatcacggtggctcccatttgatcagcctgggacacgtgaatgaatggctg tcattgaagactctaggcagggtgaatagtgtatattacaaagcgactggagggacaggc tctggccagcatggttgactggttaacacccaaaactcagaagggaagggcttgattctt tttttcttctcagagaacactaccgtgaaagcagagtctatgctgtgcataattgtgagt gaccaaagcctggtagaatataccttcactccttcatcccattattgtaattaaagatgg ctgcggggcaccaaatggtgcagggatggcctgaaatgctcttctgaaggccttttgtta agatagccaacctttcccttttggctcacagcgaaagcgtcatccgggttctcattgact tggtcctttgtgtgaaccctgtctctgattaccctctacagactcctcctcgtgggtttg gaaaggatggtgtgccataagctgttttcctttcttcaagtttttctccctaatgtactt tcctgtgaatgatcagtaatttcctgttacctgtttaagctttggtttgtgaagctggca gcctttatttctctgagtttttcccaacctccccccacccccaccacacacacacacaca cacacacacacacacacacacacacacaccacacacacaccacacatcccacatcccccc cccccccaccacttaaatcctaactctgtagctgatattcaattaggcagaatattcaca gtgtctcagcaataaggattttcaaggattagtttgaattccctttgttaccatctgtac atcttctgactgctctataaaataataattaacaataataaacaagacaaaaactgtacg tctgtctgagcccagctgtcctgggacatttggtaattttagttcttgcaacagtgatgc tagCCGGCAACTCATGAGGAGAAPrimer
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