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Human element [ hs1423 ]


Position: chr2:172,947,779-172,949,246 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: METAP1D - DLX1

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
CCTGTGCTACCGCAAAAGTCtttgatccttttccagctgtggacacagagcacccaagag
actgtcgcgatgtgcggccgtctctccccttcccccaccttgcaagtctctggaatacac
tgtccctaggcggcccggccgtcgggtttcctcacgccttgccctgagctccgggaatta
ccaattccgctttccccggtcccaaccccgaccccgcgcccgccgcggcgatcacttact
tgggcaaggccctcagggcacgcgcgagagacctggggcccggcagtatccgagcgactg
tgctgggcacacggcgtggcactccataccctaaaacaggcactaggaaggagcaattaa
aaccacaagtgcgacacagaaatcaggacgagccgagcggctcccggagttgcctggtgg
cggaatcattagctggctgttgctacaaagcctcaccgtgtggttcggtgattaagaaaa
aaaaaatcttttaaacgttggcaaacccgttattggtttggcgtattgggttttttttgc
ttttgcttttctttttgctccaaccactctgcctgaaatatttctgcccccagcattgtg
cccttaaaccaaaggagaggaattctgcgggcagcagcagctggtacagaagttaaaaaa
aaagagaaaccccctctactaatatctgtaacgacagcaccgatgtaatttgttttgaat
gttcatttttattggtgttccctctgccaaaatgtgcttgattacagaggtttgtcctgt
gatcattgcacactccagacaggggaaggtgcagcgatgaaggttcagctcaggccagat
caaatcccgggctgtttaacgctgaaaggctgcatgttgctattagtgttaaatatatgg
ctaattatgcgtatgaaaattaaacatcagtgttatgctaatggggcgccttcagctgcg
gatctgagcacttgagactaccatttaatcaaatgaatcagtaactaatacatctgcacg
tgtgaactttcacaagatcatttccccgttcccgtcacaccgctaaattatgaaagaaat
aattatcaacactttctaattacctaacaaagtaatttgggattcaccgtggggctggcg
ggatggggaaccaacatccacttacagccagggtggtgtacgccacaccgcgagggactg
gctgtattggtagtagtggcgatggggtgggggctcttgtgcacttttttgtacctagca
ccccagaagacagaaggagcagcggcagatcttgcctccactagccctgccccctaggcc
tggtggcgcgcgctttggccactggaggtgaggaagatcctgggtggcaacgagagacgt
ctacacatagataagggagcctaccacaggcctgggtttgctgcgggtttcggtgtcccc
cagggactCCTAAACTCTGGAAGTCGCG
Primer
(+)CCTGTGCTACCGCAAAAGTC
(+)CGCGACTTCCAGAGTTTAGG


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