Mouse element [ mm1412 ]

Position: chr13:15,761,774-15,763,030 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: Gli3(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.

Fasta sequence

GTCACGCCTTCAGTGTCAGTAGgggtttatgaggattatttatttatttattcatttatt
tcattgtttaatgtttcccttcttgctgcactttattttccagtttgcacttagagcctt
gagttctccaaacgtcttatgttactgcacaggattttgtttatgtctcttaatgacaca
ccatctccctatatgccaccttgcttttttgtttgatacaaagtggtgctcccatattct
agagagcaggacaggatacacagcaaagtggccctggcttatgagaaacaggtcttcctt
gtggcttccttgtgtgaggtgttgaatgttctcacaccttgctcagtacagtttccatag
caagataaagcagtcgaggcacaaaggaaacacagcctaatggtactcctccttgtgttt
agcagagataaacttcagtggagtctcagtgctgagccgtttggctcctggcagcagctt
taattggtaaaatgcagatttgatttgatttttcttgatcaggaggggattattttgtta
tcagtgcagagcatcttatttcaagatgaaaagcctttacggaattacagatgccagtgc
ttgtacaaaggttttgttggcgattgacaactcaaagagaaagtatgatttattagccta
taatggctaaccctaagcaatggtctaggtctacagattgtgaaattgcttatgtgaact
gcctttttatgccactcatgcccggggttacgcaaataaatgtagagtctgcactacaca
gaatgtcctaagagaggaagaaagaaatgaaggtgggggtggggaaaaaggtggtttcta
atatccatcttggtggtgccttttacagtagtttggctgataagcctgcagaggaaagat
taataacgaggcttgtcgctatcagtacctgtttgtcgattctctgtaacagggctgtct
gtccagcatggaaattattgattaaataaaaattgctattagattgtgatgtgatccata
atcacaaacaatggtggacattcctctttcagatcttcagccatgcatcaagttgttgcc
tttccccctttgccgtaatttatattgtgctgacatttggactcattaggtttatccttt
ctgcttgaagcacaagcagggtgactgctggacgagggggaggaggagccagacgaggac
cagccagggcagaaacctggctgctgtgttccctgGGGTAGCATGGGAGCTACTTAA

Primer

(+)gtcacgccttcagtgtcagtag

(+)ttaagtagctcccatgctaccc