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Mouse element [ mm1255 ]


Position: chr14:123,270,776-123,271,899 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: Pcca(intragenic)

Timepoints:
e12.5

This element was tested at e12.5, but did not show reproducible staining at this stage.

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TGGGTCAGAAGCACATGCATcaagtaactcagaagggactctccttaaccatgagttata
gtccagatgtcctggctggcccacacattctgggatttataaatgcattttcagattcat
tcaacatactcgatatttgacaggcagaacttttaaaagaaaattgtgtttgtaagaagt
aaagttattttttttttcttgtctattgctgtacttgaaggattcctgttcataggcaga
aaacagaggtttcaaaggaaccttgtctccatatggttttcctcgataatcccttatcct
ccatcttcagctctcattcctttgctttcttgcctgtggctacatttctatctctgcatc
aattagcacagtgatttatacaacaagcaagcagtttagaggtaatggtacacaattaac
atctcattccaccataaagggtatgctaaacaccccagtcattgctttctctgcctggaa
ttgaaataagtgtttgtcctaattcataagctatctgatgtgaagtgggtccgctttgta
ctgcactacaccattaatctaattatagatagctgcaaatgaagtgctgtcggggctggg
agattgaagaaaatagccttttgtcatttagcatgactctgtgaagcaggatcacacttt
atagggtaatgctgcaaaatggaaatctcaggaatattgctgcctgttgcatcttctcta
attactgcagcattgtaaacacaactgggcgctgatataaatagagctgtagctatgctg
tcatgtgggttggaggagaaagtccttcgagtgctgttggattaaatgatgcatcctgat
tgacagctaccttgagactgcactgatggagttgtcacagaactatgctaacaggctgag
ccaccactgcagctgcaggaagcctgggctgagactgggctggctgggtattggcctgtt
gctttagctgcagcctcctccttccaccttggttgctgactgaaccttgatgaagaagct
accagctcctggtctgtgtgttttcagtatgttttctggagatttctctttcaaaagatt
agctaataggctctgggttcaaggAGAGAAACAGGTGCAGTCCG
Primer
(+)TGGGTCAGAAGCACATGCAT
(+)CGGACTGCACCTGTTTCTCT


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