Mouse element [ mm1235 ]Position: chr5:132,000,566-132,001,785 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: Auts2(intragenic) Timepoints: e12.5 This element was tested at e12.5, but did not show reproducible staining at this stage. This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence AGCTGAGTGAAGGTTCAGGCaccatgaaattaataaaatcaccatctcaccctctctatt gcaggttcaggaataaatcgggagaagaaatctgtgaggcacagagcgacctactcaagt gtgaagcaatcattttctccactgacgtcttccagcgttttcttccacagtgcatttaca ggattcctagttcaccaaagttgcttcctaaggctgaccagcactggatttttttttttt tttttttttttttttgagtcatcgagcccaattctgtttgctaaataaattccgggcaag agtgctctgactcttgcccaggcaaggcaagggtctaaggtaagccataaaacaatagtt ttatcttatctgagggacccactgtattccggtgtgtgtgtgtgagtgcctgcgtacatg tgagtgtatgtgtgtgtgtgcacgcggagcctgtgagtgtgtatttttactgccaaaata aaataaatggaatcggcacacacatgataacgcactctttgctaactcatactaattgct gggctaattgatgtttgttgaactttctccgtttcattgagtgattaggtacaaagctaa cagttgacatctgtaggcgcttaactggcgtctgtaattactgttaatgttaaataattg ttagttaattggagtggtaactgactgaatggaatccagattgggaagcagaaaagaaac ctagttaaataaacatacattttttgggggggagaagacaacggttttttggtttgagtt ttgttttggtttggtttttttcttgcaggtgtcagaaactagaaataacccagaagcctg ggatagcccaagggcttcctgaatgaaggaaacccagcctggggcggaacaatgtgttag aaagcctcacttaaagagcaggcggctttggtggctaacccctctcgctgcaggtctaca acacagacttggtgttttagttgtaataattgctatcacaatggcctatgtgaatgtatt cctctcacacccagacacccagacaccagaccaatgtctaaaggaaacactacatacaca tacccactgatggactgaaaggggcttgcagaacattcaagcttttctctgaaaaccact gggtatatgggaaacgatttcagacaaaggatacagaggtcacccaataaaggaagtgaa GGGCTTTCTAGTGGGCTCAGPrimer
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