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Human element [ hs1221 ]


Position: chr10:119,313,709-119,314,860 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: EMX2 - RAB11FIP2

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
AATTCCACCTTCCTCACCAAGTcaaacctcaccttccgatccctatctcccacccagtgt
tcaatctccacatcgagcctcgccatcctcctccctaccccacacttactgcccctcctc
ccattcccgagagacagtacagaagtaaggtctccattcccagcacaggcacaaaggcca
agatgcacctgggggtcctgaaggtgcttgggatgggcatgcggtctcatttggaaaatg
acttgaggatgattggcctcgcctggcggtggcctggctgccagcaacgtctggatggct
gggcctcagcctggccccagacgtcccctgctcccagccagctccaggccctatatatag
cacgggtctccatagccctggctggaccctgacgtcagaaagaacttgatcttgcctgct
tcgctcctgcttctgaaactgatccttgaaatgagagaacagactctacacaattcccat
ggccttgacataaggtacagcgataaatatacaccactaatgagcaattaccatataatc
accttccaattagctcgcataatgatgaattaaacattctagcaggcgggattaggtttc
ttactttgtatattatttacgaaggctatagcttctgcaaagaaccaaatatcaaattag
atgggggaattttcacttgggggtaattatgcattcaggccagcggctgtgttctggtca
cttgtcaactgaggcctcctgcaaggtactgaccagtgcttttgggcatttattttcctt
tttttccctctctctctttaagttgcaattgccttttccttttccttttactactctggc
ccgtgaggtgagaggctggggacactgaggcggggcactaggtggggtttggggagggac
ctcagagaggatgtggtgcaggcatcaggccctgtccttctggccccattcgcttggctc
ttgttcccgtttctctccagcccaagaaggatgcctgagcaatcggctctgcacagctac
tgagcacagggccagcgcggctgcccactcacctaatgccaggggcttcctcccgctgca
cctggtgccaccgcctgacattccttaggcccccccggggcctcagtgcaGTCTATGCCA
ACTTTTACCCCC
Primer
(+)AATTCCACCTTCCTCACCAAGT
(+)GGGGGTAAAAGTTGGCATAGAC


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