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Mouse element [ mm1000 ]


Position: chr1:170,346,159-170,348,567 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: Pbx1(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
atCCTCTACCCCAGGAGCACAAGgaagggaaagatcctatggagcttataatgagcctag
ttcttctgtggactggtcagacactaggctcttgaaaatttatggagagaactaagtcat
agatatgtttcctagttgtgaggcacaccaacgtggctggcttctgcctgcccacaagaa
tgcaatgctacgtttccggggtaagggggtgccgccccaccatgctgtagatgggcaatc
acagaaaagcacacagatccattgaagaataacaacttcacgtcaagtgcatctttccaa
gtgtaactttcgaagaagaaaaataaagtccagggactttcagcagaagcctgatgacag
ccccttcctgccctggctccagccagcttggtgggggaggggacacaactgtgttcacag
taccccaaagaagttgtccgggtttctaaaaactggcaaaaatcaggtggctaaaggcct
ctcttcacacaagtctctgaaacaaggcaaggggacatacaagaaactaggtagcataga
agtcctggagccttctctgagtgttcaagaaatcacaccaagcttaacaaatgtcaaagc
tgtttaaagtacatcaaggttcttagggttaaaaatttccccagggtaagtgctgcactc
cccataatagctgagagataaagaaggcaggatgggagggatttcagagtaactccaagt
tttgcgatttaatccaattcgatagatgaagcaacaactgggctatttgttgatcagaga
tgaaacagaggcaactaaggaaagagctgcccctaccccaccccctaccgaatctcactt
ctttatgcaaaacctcctccagggttgaagcgtgcatttgagaaagcagcccccactgct
ggaagcccaaacagcacaggcacagcccagagtaggaagcctgggggccctgctggcatc
atcggggaatccagccccacactaaaagtgttccgtaaaggaagcaagactccagagcaa
gaggatgctttccaggaagaccgttctcctgtcacccttggggtcagcctggaggaatga
gaggagcctggcctgcagtgctcccagccgatcctcggcctccagggccaagcaccgggg
tagcaaagttcagctcaagccttgcagatctgcactggggacttgagacaagaccccgag
agtgaccctgagtgtctgcctaacagcctccctgtgcattcccagctgctgcagggctgt
accagggctagtctgttgtggatctgtgatgactcagccactacaccctggtgcctctgt
taggctcagatcctcatgtgagattgtgccttgggaggatctgtcccctgagcaccaggc
tcttcatcaaactcctttaccaccccaacttcctccaacaccctcaatttctcatttctt
cccctgattaaggcaaaaaacactttcttatgtcttgaatattcttatttattgttgggt
gctctctctctctctctctctctctctctctctctctctctctctctctctctccttctt
tattcttcccctcatcccctgcaaaggattcagctccccaaaccacaccatgacttctta
ctcctatctagccagcatcaccactgaccgatacctacttgggatttatcctcctaaggc
cttagagatgattacagactccctagcaagagggccacacaaaggcgagctgagcccagg
tgcctgctctttgttttaactgtgtgttcatgtgggctgctaggaggaggctccagcctc
tgcttgccctgtgtggcaaagaattagctctgggctctcagagctagctgctcccgcctt
ccagaaggatgccagctgggctcagccgggttctctcaaggaggctttctttgttaggga
cgaaatataaatgccaagtttcagccctattaacagtggtaattattagaaatctcctga
gagcggcaacacttgagatctcaaatcacccaaaaccttcagtggaaaaaaaaaaaacaa
cctgctactcttttccaggtttagtttagcccagaccaccaggtaaatgtattcctttga
gctgctgctggcagattcaactggtctgaaagcaagacaaaaatgtcaactttccttttt
cccctggctgttaacacatttgcaggtaaaaccaagcatctttctttttaaaaaaaagta
ataataataataataatcaaaaacaaaaaaaaaacaaaacaaaaaacaaaacaaaaacca
aaaaaactcactttaataacatttccaaaacgtttcactgacagttatgaggaatgacag
aagatcaccaagtcacacgacaggtctatggtggaagcaaactcaagacTTCCAAACTTT
CTGGGCTTG
Primer
(+)cacCTCTACCCCAGGAGCACAAG
(+)CAAGCCCAGAAAGTTTGGAA


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