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Human element [ hs647 ]


Position: chr12:18,229,480-18,230,430 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MIR3974 - RERGL

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TTGTGTTACATCTATATCCAAAAGGAAgaaaaataatgtttggtggtttatatatttgaa
aattatgatgaatttttttcttttcttatgaaataatatcttgtcactatagactatatt
tcaaatttatggtgtgcctctatttctttgggaatttaaaatactttaaataatttttgg
gctttgcctgattccccagaattgtagagtatgactgataactgcctttagaatccttaa
tagctaaattacaattattatgcaaagccctacaatagacagttatatggattgttaaat
gactttgccttttttgcaaactataatcattttcctttattagctgaaggattttataat
ttaaggaagatgagaaacctataagtcacgctttcaaataaggaacatggatattgcaaa
cattaaattgccccataaaaatgttaaggcatcattaaactaggttcagattaatacaat
tcagtcaactctagatagatatctcccataataagctgaaacaatacctagtgatctaag
atgtaaaacattttactacagccaatttataaacctataaaacatacaggtatcattcaa
cctctttattaatagtgcatgccttgtattaaatcaaaaccactttaaagacccctcccc
tttggcattcccttcctcagctgttgcccctcattatatgtcatctcactgaagcttatc
caaaatgaatgacagcatctttcttactagaagcagcataggcaattcccagcctagtga
tacagcttggctagctctgagatttacagacacacttgctgtcctcaaactacaacattc
tattgcttagtgttgatgtatttgtttctctgtaggtatatgtggtttttgctcacttta
gaaaaatgcaacttattatttttaaGGTTTTTCAAGTTGTTTTACAAGTGA
Primer
(+)TTGTGTTACATCTATATCCAAAAGGAA
(+)TCACTTGTAAAACAACTTGAAAAACC


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