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Human element [ hs345 ]


Position: chr14:34,068,299-34,069,069 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: NPAS3(intragenic)

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.


Fasta sequence
TGGGAAGAATCAACCCACTCtcaagtgggcacatttccatatttttgcatgtccgtgtat
ctgtctgttatgtttatgtgtccgtatttatttgctccccaacctgtaagaagttgaacc
gtttaaatccatttgttacctgaattgttttctcctgttttgaaacattttaatttataa
tcagacaagaagaacagatgtaagagttgtaatttaatcattttattgctatctttgtat
attttgaagaattaggtattgtactcatgggaagtcgtattttatgcctagttggaaaaa
tgaataatgtaataatatgcaaaatgtaaagttttagaacttcactactgagtaaggcat
ataaactaatgatctggttaaggtcccctgtgcaccataataaatatggactaaatttgt
cattatagctttagacagaggtttcaggcaacagatttaattaaatccttttttaatatt
catacttctaaactgcataattcccttctactgtaaaactgagtgctatattaaaacaaa
caaaaacggcagattactaaatggatattagcgtgaagtcaataagcatttaattctgga
gagaactctgtgaggagagaggaagcttcatggagctcattcaggagatcttgacttaga
tttgggaagctcgttcaggagggcttacttctgttttcatgctgactcttgtaggggagg
ttaattagttaattttggaactcataaaggaCAGGAGCAACAAAAGAAGGC
Primer
(+)TGGGAAGAATCAACCCACTC
(+)GCCTTCTTTTGTTGCTCCTG


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