Human element [ hs254 ]

Position: chr3:114,574,745-114,576,011 (UCSC browser)
Source: Lawrence Berkeley National Laboratory

Flanking genes: MIR4796 - GAP43

Timepoints:
e11.5

This sequence did not show observable enhancer activities at E11.5
We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution.

Fasta sequence

aAtaGCAGCTTCAAAGCGAAAGTTatgtggcgagagtaaataacccaaacttaacgtgac
cttcctagcattattttagggaggcgttagggtatagagagtgaaaaacaccctgaggtg
ggatttacaagtcttaatttcagtttcagcagtttcactttctggctgtgtggtattaga
caagtggcttaacctctctggacctctctgatccttcagatttaaaatgagggggttgat
tactaagtgacgtctaagagcccttctagagataaaaaattccccaatctatgataataa
taattaacatttgatggtcctttaccacctacaaatgatttccacatacattatctaatt
tgatcttcatgccagttctgaggtagtattattatgattatgcccatttttggaagagag
attcagaaaccacaaaatggcagagttggcattgaacagatgttcccaagaggcaaatac
cccttcagtaaaagaagcagctgatgctaacaaggataaataagagcagacaattcagcc
tgtgagctcttgtttaggggtacaacagaatttctggaagctgcattggggtaatgataa
tccaccatgtggaatgttatctgatgatgtatttatcaaaaaaaactaataaaaagaaaa
ttaaaaaaagatacaataattgttactgcactacaccctaatgaattattgtgttcaagg
ttcttacaaaaaaaaagtaatattatactcggcttatgttttaattgatttattaatatt
ttatcacaggaatgtaatttaagctcctcatgatttagatctcataaacctttagcattt
tgcaaattcaaattccaatttaaagccagggcgagcaattaacttttctatcatcttgtc
aggggagaaggttcgtgtttaatttgagacaaagtgacagaaatatatatacactgtatg
tattactccaatttaatttatgagtcagcaccatccaccttcaaaatgtagatttaatag
aagaggaggaagaaaaggccgtgctgtcatgatagggctgtaatatctgccctgaatttc
ttacatattaaaaaaaagtcactggtttgttgagataccaagctagattgattgcaggcc
tatttccagttcttttgtttcacattccacttcctgattcttttctcatacagcacataa
tctcatggatatcacagggagttcttacccccacttctacctctactCCTACCCCATCCT
CCATTTT

Primer

(+)CACCGCAGCTTCAAAGCGAAAGTT

(+)AAAATGGAGGATGGGGTAGG