Human element [ hs148 ]Position: chr2:45,066,752-45,070,624 (UCSC browser) Source: Lawrence Berkeley National Laboratory Flanking genes: MIR548AD - SIX3-AS1 Timepoints: e11.5 This sequence did not show observable enhancer activities at E11.5 We define a negative enhancer at this developmental stage when at least 3 transgenics were obtained and yet no reproducible pattern of expression was observed. This does not mean that this sequence is not an enhancer at another time point or a weak enhancer at this time point but below the detectable range at our level of resolution. Fasta sequence CAGTCTCCAAAGAAATACCCactgaggcgcttgggtaccagcctctggcagccttttagc tcttctcttccatgccttccattttctcctcccctggaaaggcacctgcagccctagcag aaacgtggaagtcaatttgtgtggctggtgcaagcgttttagcatattgagtggtccaaa tatttaaatatctatgatataggaagcgtggagaacattaaaaaccattttctccctgct gattgcaagagaggaaatacacacagtcctgctctcctgcacagttgcaaaactgctggc ctaatcatattgcctacgtaaagaacctctcccgcctccacgaatctggctttaggttgc atttactgtaaacaaagatgtatttggggggggcagttggattgagtttattgtttatgt aaatctctcagaatgtgacatagcaagatagcttcctatgttgtgttgatccacatttta ggccaacagccagagtattttatcttttccttcattatagtgatcttccaattagccgtg gtctgtaataccctgattttggtggcagtggtttggacaggattgtttgatagttctgct cgagaggcaaaataaggggccttattatagaagtgcagaggttgtgcaggagctgagttc tgttgcaaagtttgccgataaggggaaaaccggcccttaagcgtccattctgttctgtat attaacacttctaatctgcactgctgataaaactgcaagccatttgcctttggaggaatt agttctgcctcacttgtgtaaccaggcgatggtgattaacctttaaactgtccaatattc ccagcagcctcctgctccagattagaaactatttagttatgaccaactaaattaacaaca ctcccttctctgaagaatgagatttggaaggtggatgcattttaaggtggaattgtgttt tgaattggtgatccatccttctagggtgtttatttcctcatccttaaagttcaggctgcc acactgggcatggaaggctcagctggggagtggggaggtaagtagaacgagagggagacc agttgtcctggttggatttgtagacacaaggttttatgggcacaaaagaaacctgccttg ggttcagatttccttccttgttatcatcccaggtgaaaaggggacattagctcttctgca agacccaaaccatgctcacagtgggactgagcagttgttgctaatggggagattaaggaa taaataaagtagctcccttactcccaatttcttcctggagttgaatgtcaggaaaagagg cgctaactaactttcatgttccagacatagggatatgcccaccgcaggtgggtctaccca gttgagctgcagccatgctaacaatcctggttcagccctctcctatctgcccatttcaaa tgtgtaatttatcagttgaaacataccctcggtggtgagggccaggcaattatgtaatcg tgtcatgccttaaatccccagattgttggtcctccctggataacaaataaaatggagcag caatcctcaccaccagtaccccaccaccacccaatgctggtagcgaccttttctgtttta ccagtttcatattagctacctccttaagaactgctaatctagatactgcattagcaggat gtcagtcaagccgtagtccacggcaaagccttcttcatataccaatttaaatcattcatg gtcttttctttggcattattcaaaagatcataataattttcttgtgcccacagcacaaaa aaatcaattatttccccacaaagacttgaaagtacttagcgagtgcggcaagaaaagctg agggtgatatcaaattgaaattgcactcattacatacaacacaattagttgccactagcc tggccgggctttaattctaaatggcatatctttattattacagaaacaagcatgcaaatg gcattgttcaggatttgccgaaatcaataggatatagtgctgtgtgtttggtggccactg tcactcacgctgctgtggatgcgagataatgtgtgtgtttgaattaatgaagggagcagg caacatcataagtgacacccaaataaaagaaatgggtacatgatatttgtaattattcta cttgtgtttttgttgtaaatacttgtactttttgttttttaatcaaaggattaattgcat tttaataaggcattatgatttgcagagctttagttaattaaaacttgtagtatataatta tgaagtgatgctgagattttctaaagatcacctatatgtttagaatatgtataatttttc tgttaagcagtggcaacctcccctccacccttttttccttggtccctcccccagcaacgg aagacatggatgacttacgtgaaatgttggtgaattgacaattctaggtggatctctgta agtgacaaatagaacccaatttaggttattgcacaaactaaaatgattctgaaggagcac tcatgtgataaactgtagcagaaaactttctccttgacacttgccttgtcgttaatcttc ctgagatgtagcactatagaagcttggggagttgatgtgagttttagagtgcaaacattg agattttagacctgggtgaggtgatctcactgagttggaatctaattggttctctgcagc taggccaaaaccccggtggagtcactgaaccccagcttgtcctctgtgacactcaagagc ttatttgggaggaatgggatggggagaggggatgctgaaagtcaagggagcttggaattc cctagtgttaacacattagtaattcacagtggaatccagcccagagatgtgtaattgcgt ttcctgtaggtgtgtattatgtgaagactgatggattaattgctagatagatggaataga gctaaggtaaaacattattaaaaagttttaaaatcttaatgtaacattttgaagtttgct ttggggattatttttcaactcagtctcctactttccattctctctgggatcaggagtggt agttgtctttgcagaaggaggggtccatttgttgagagcttagtatgtcctttgtgaagg tcaggtggccctgtgagggcaggtggatgcgggagctaggagctggatctcgtgtggcca gtgatgtgacgggatgagctgactgcaaaagcctggactccagttaccctttggggtatt gcctggacaccaagggctaattattccctccctataaacacccagatgctgcaatgagta gtatcaagaatctcccccaaccccctgcaagacaaagtgaaagatgaagttgaagggaac atcccacgatgttcatgtcccaagcctggttccctgggatgcccacaacagtgctctaag tagggtggcccaaagctgatttaaaatctaatactctcaggttggtcatggcttcctact ttcactctgaaaacctagagcttgaaaaggtggttcattcaaagcttgctaaaatatttg ctcagcagcacaatggtgtgtgatggaaaataagctcttgtggcttgcttcatcctatca gccgcggcctgcagcagggaaagaatgaggggaggtcagtcaattatgtgtggtaatcac cgtaagcatattatctgagacacagatgtcttctatgaccccacacgacatggctattaa cctcggagcaatataaaaatggagtgggctttgggggaggtgtggaggtaggccagactt tcaggctagccttgttcacaggctcagaaaaacaagttctggacacgaagactattttca gaaacccatcCAACAAACTGACCCCGGCTCACTPrimer
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